Journal: Cell reports
Article Title: Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy
doi: 10.1016/j.celrep.2020.108303
Figure Lengend Snippet: (A) Representative images of 10-week-old, Nissl-stained coronal sections at three matched bregma levels from WT (left) and Kcnt1 m/m (right) mice. Scale bar, 1 mm. (B) Schematic shows the experimental approach of recording glutamatergic neurons in layer 2/3 of the motor cortex. (C) Representative membrane voltage traces of glutamatergic WT (black) and Kcnt1 m/m (orange) neurons in response to −100, +140, and +240 pA current injections. (D) Individual neuron values, mean, and SEM of the R in and rheobase of WT and Kcnt1 m/m glutamatergic neurons. (E) Number of APs (mean ± SEM) per current injection step in WT and Kcnt1 m/m glutamatergic neurons. (F) Schematic shows the experimental approach of recording NFS GABAergic neurons in layer 2/3 of the motor cortex. (G) Representative membrane voltage traces of NFS GABAergic WT (black) and Kcnt1 m/m (green) neurons in response to −100, +100, +180, and +300 pA current injections. (H) Individual neuron values, mean, and SEM of the R in and rheobase of WT and Kcnt1 m/m NFS GABAergic neurons. (I) Number of APs (mean ± SEM) per current injection step in WT and Kcnt1 m/m NFS GABAergic neurons. The lines in the current/AP plots are fits to a Boltzmann sigmoidal curve that were used to determine the I 50 . Statistical significance was tested using generalized linear mixed models. See also .
Article Snippet: Whole-cell current-clamp and recordings were obtained from cortical layer 2/3 pyramidal cells and interneurons at 32°C using a Multiclamp 700B and Clampex 10.5 software (Molecular Devices).
Techniques: Staining, Injection